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That effect is observed on different cell types, with differences in the amplitude of cell index decrease, suggesting that the effect of glutathione is not cell specific but likely widely present in all cell types (and not related to cancer cells)
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However this technique has some disadvantages such as: this technique relatively high cost, leakage of ligands may accur from the column and sometimes the desorption procedure results in partial denaturation of the bound protein [24]
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