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The persulfide anion is expected to react faster than a thiolate of equal basicity, according to the alpha effect ( − acting as nucleophiles form products that contain two electrophilic sulfur atoms (Figure 1), which make it possible to recover the thiol species by a subsequent nucleophilic attack to the original outer sulfur
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To surmount these challenges, protein engineering and enzyme immobilization techniques have been proven to be effective in enhancing the performance of PET-degrading enzymes, such as improving their catalytic activity, thermostability, and reusability
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